The rate of meiotic divisions was monitored by DAPI staining as previously described [41]. Cells were pelleted by centrifugation (~ 2400g, 1 min, room temperature) and fixed in 80% (vol/vol) ethanol for at least 60 min before further processing. Subsequently, samples were pelleted by centrifugation (~ 2400g, 1 min) and re-suspended in PBS with DAPI (1 μg/ml). Cells were sonicated for a few seconds and left in the dark at room temperature for at least 5 min. After DAPI staining, the proportion of cells containing one, two, three, or four DAPI masses were counted using a fluorescence microscope.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.