MRI scans were acquired along the length of the right tibia (for triceps surae muscles) and femur (for quadriceps muscles) using a magnetic resonance imaging (MRI; 1.5 T Signa HDx, General Electric, Milwaukee, WI, USA). Participants laid on the MRI table on their back. The ankle was held at 90° by a custom wooden square to ensure the same position between participants and test sessions. A specific antenna for the acquisition of muscle cross-sectional areas (CSA) was installed (covering 45 cm). The femoral quadriceps and triceps surae muscles were scanned using a VIBE (Volume interpolated GRE) image sequence (Matrix 240 × 240; TR 6.3 ms; TE 3.0 ms, cutting thickness 2.6 mm, gap = 0). An axial scan was performed perpendicular to the thigh, from the femoro-tibial joint to the iliac crest for the quadriceps muscles, and from the calcaneus to the femoro-tibial joint for triceps surae muscles. Due to the length of the antenna, the quadriceps scan had to be performed with two sequences for some tall participants (n = 14). In this case, external markers (oil capsules) were placed on the participant’s leg using adhesive tissue to be able to adjust and overlap the different acquisition sequences in post treatment.

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