Thick and thin smears were stained with Giemsa and examined under a 100× oil immersion objective by level 1 or 2 WHO certified malaria microscopists. Apheresis samples were expected to have a significantly different composition in terms of proportions of RBCs and WBCs when compared to whole blood (e.g. RBCs make up 1% and approximately 46% of 1% HCT and whole blood samples respectively). As such, standard parasitaemia measures by microscopy were not feasible. It was decided that the expert microscopists would estimate parasitaemia based on sample composition.

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