The size of lower limb muscles was determined from multiple MRI scans before and after a 12-week resistance training program as described in detail below. To prevent an influence of fluid shifts on muscle volume, the MRI scans were performed at the same time each day [31]. Two pre-test scans were performed (4 weeks and just before the beginning of training) and the first scan was used as a ‘control’. Post-test measurements were performed 4 days after the last training session. Values of each parameter obtained during the pre-training sessions were used to investigate the effect of aging.

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