Eleven recreationally active young men and 35 sedentary elderly men were recruited to participate in this study. The present study is a part of a larger study, designed to evaluate the effects of training intensity and age on changes in tendon architecture and its mechanical properties, lower limb muscle adaptations, and motion capacity. This sample size was determined by a preliminary power analysis (Continuous outcome superiority trial, α = 0.05, 1-β = 0.90) and performed with Sealed Envelope software (Sealed Envelope Ltd. 2012, London, UK), based on previously published data showing significant increase in cross-sectional area (CSA) of the muscular and tendon systems by MRI using a similar training protocol [7, 12, 2428]. With these parameters the required population size had to be composed by 12 subjects in each group. Their physical activity level was primarily determined through their leisure time activities assessed by a PASE questionnaire [29]. Only participants with a PASE score < 150 were included. Each included participant completed a medical questionnaire and was examined by an orthopedic doctor. Briefly, all participants were healthy normotensive (< 140/90 mmHg) and non-obese or anorexia (20 < BMI < 28), did not take any prescription medication, had no overt symptoms of diabetes, atherosclerosis, or any joint, muscle or tendon pathology, and without metallic implants/objects. All of the participants were fully informed regarding the experimental procedure and gave their written informed consent. During the training intervention, participants were recommended to abstain from other physical activities. The experimental protocol was approved by the local ethical and personal protection committee (CPP-2016/52) and registered on ClinicalTrials.gov (NCT03079180). The participants were free to withdraw from the study at any time. Our study adheres to CONSORT guidelines [30].

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