Parasitaemia was quantified using 18S qPCR targeting the highly conserved Plasmodium 18S ribosomal RNA gene [16, 19]. Quantitative reverse transcriptase PCR (qRT-PCR) assays were used to quantify gametocyte levels with assays targeting the P. falciparum pfs25 (female) and pfMGET (male) gametocyte mRNA transcripts [20] and P. vivax pvs25 (female) gametocyte mRNA transcripts [21].

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