A Zetasizer Nano-ZS dynamic light scattering (DLS) system (Malvern, Montréal, QC, Canada) was used to measure the particle hydrodynamic diameter of the isolated exosomes. Each sample (100 mL) was loaded into an ultraviolet microcuvette (BRAND; Essex, CT, USA) at 4°C. The Brownian motion of a particle was measured by the fluctuations of scattered light intensity at a wavelength of 633 nm and a fixed angle of 173° to indicate the velocity distribution of particle movement in solution. The diameter of the exosomes was measured using the Stokes-Einstein equation to determine the particle's hydrodynamic radius. Each data point from each replicate represents an average of three measurements of 12-18 runs, which was set automatically. The average particle diameter was obtained from the peak of the Gaussian model fit to the particle distribution. The polydispersity index (PdI) was determined to reflect the width of the primary size distribution in solution 22.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.