At the end of measurements, the ventricular tissues were quickly removed and fixed in 4% paraformaldehyde, embedded in paraffin and sectioned at 5 microns. To identify the pro-inflammatory M1 macrophages, the ventricular samples were incubated with primary antibodies against CD68 (1:100, Abcam, Inc., UK) and inducible nitric oxide synthase (iNOS; 1:50, Abcam, Inc., UK), and cells were stained with DAPI for nuclei. The results were analyzed by Image-Pro Plus 6.0 software. More than 3 sections of ventricle from different samples were randomly selected to quantify the number of macrophages.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.