At 16 h after surgery, 0.5 mL of whole blood were collected from each sham-operated and CLP-treated mouse into tubes containing anticoagulant. After incubation at room temperature for 15 min, the samples were centrifuged at 3,000 × g for 10 min. The white blood cells were slowly removed from the corresponding layers, and serum samples were extracted. Exosomes were isolated with the exosome isolation reagent, ExoQuick-TC (System Biosciences, Palo Alto, CA, USA). Briefly, the supernatants were transferred to sterile tubes containing 63 μL of ExoQuick-TC Precipitation Solution (System Biosciences), mixed, and incubated for at least 12 h at 4°C. After incubation, the samples were centrifuged at 1,500 × g for 30 min at 4°C. The white pellet containing exosomes was resuspended in 500 μL of buffer.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.