Fresh culture medium was replaced when the density of Caski-FRA1/Caski- empty vector and Hela-FRA1/Hela-empty vector cells in the 6-well plate reached 50-60%, and LPS (50 ug/ml) was added to the cells for 24h at 37°C. Whole cell lysates were collected and the ATP levels in the cells were measured according to the protocol as defined in the Enhanced ATP Content Detection Kit (Beyotime, Shanghai, China). The ATP levels were measured using a SoftMax Pro 6.4 microplate reader (Beckman Coulter, USA). Cell concentration was measured to normalize the level of ATP.

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