At 7 and 15 days post-embolization, the rabbits were sacrificed, and the infarcted tissues containing embolic MS were resected and fixed in 4% paraformaldehyde for 48 h prior to evaluation by histopathological analysis. Thereafter, the central auricular artery and the surrounding tissue were dissected, embedded in paraffin, and then cut into 5 μm thick cross-sections. The sections were further stained with hematoxylin-eosin (H&E) and Masson's trichrome, and examined under an optical microscope. After 15 days, the morphological and ultrastructural changes in the vascular endothelial cells in different treated groups were investigated via FETEM. The details are presented in the Supporting Information.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.