LPS (50 ug/ml) was added to Caski-FRA1/Caski-empty vector and Hela-FRA1/Hela-empty vector cells for 24h at 37 ˚C. Cells were lysed with RIPA lysate buffer containing 1% protease inhibitor. The protein concentration was measured using the BCA protein quantitative kit (Lianke biotech, China). Equivalent quantities (30-50 μg) of protein were separated in 10% SDS‑PAGE gels and electroblotted onto PVDF membranes (Millipore, USA). The membranes were blocked using Tris-buffered saline containing 5% non-fat milk. After that, the primary antibody was incubated overnight at 4 °C. The primary antibodies used in this study were as follows: FRA1, SCO2, COX2, G6PD (ImmunoWay Biotechnology Co); LDHA, MDM2, p53 (Wuhan Boster Biological Technology, Ltd.); GLUT1 (Abcam). After three washes, the secondary antibody (Goat Anti-Rabbit Ig(H+L) HRP or Goat Anti-Mouse Ig(H+L) HRP, Affinity, USA) was incubated at 37 ° C for 1h. After another three washes, the expression of protein was detected with a LuminataTM Forte Western HRP Substrate luminescence solution (Millipore, USA). Then β-actin (Santa Cruz Biotechnology, Inc.) was used as a loading control. The quantification of band intensity was performed by using Image J (National Institutes of Health, USA).

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