Four-mm histological sections of renal tissue were stained with hematoxylin-eosin and examined under light microscope. For the evaluation of renal damage, 20-30 grid fields (x400 magnification) measuring 0.245 mm2 were evaluated by graded scores, according to the following criteria: (0), less than 5% of the field showing tubular epithelial swelling, vacuolar degeneration, inflammatory infiltrate, necrosis and desquamation; (1), 5-25% of the field presenting renal lesions; (2), involvement of 25-50% with renal damage; (3), 50-75% of damaged area; (4), more than 75% of the grid field presenting renal lesions (n = 8 mice per group) 20.

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