Lysates were prepared using NP-40 lysis buffer supplemented with a protease-phosphatase inhibitor cocktail (IBI Scientific). Lysates were ran on a 10% SDS-PAGE gel prior to transferring to nitrocellulose blots using semi-dry transfer system (PowerBlotter; Thermo Fisher). Membranes were stained with primary antibody prior to wash and secondary antibody labeling. Protein bands within blots were then detected using the Licor Odyssey imaging system (Licor, Lincoln NB) and analyzed using Image Studio 5.2 software (Licor).

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