Spleens were fixed and cryosectioned prior to transferring onto L-poly Lysine-coated glass slides. 0.3% Triton-X solution was used to permeabilize the sections followed by blocking with 0.2% BSA. Sections were stained with CD11b, CD64, F4/80, AIF1 or IgG isotype control antibodies. DAPI (Thermo Fisher) was used as a nuclear staining dye. Slides were imaged using the FSX100 fluorescence microscope (Olympus, Waltham MA). Acquired images were then analyzed using ImageJ (Rasband, W.S., ImageJ, U.S. National Institutes of Health) and FlowJo (Flow Jo LLC; Ashland, OR).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.