2.6. Corticosterone ELISA
This protocol is extracted from research article:
Sex differences in the delayed impact of acute stress on the amygdala
Neurobiol Stress, Jan 7, 2021; DOI: 10.1016/j.ynstr.2020.100292

Rats were anaesthetized, decapitated and trunk blood was collected into sterile pre-chilled 2 ml centrifuge tubes (Tarsons Products Pvt. Ltd., #500020). The clot formation was allowed to happen for 30–60 min on ice following which the samples were centrifuged at 12,000 g for 20 min at 4 °C. The serum was then aspirated into fresh pre-chilled 2 ml centrifuge tubes and stored at −20 °C for further testing. Corticosterone ELISA was performed using a commercially available kit (Enzo Life Sciences, US) following the manufacturer's instructions. Sensitivity of the assay was 27.0 pg/ml (range 32–20,000 pg/ml). Absolute values of corticosterone concentration were used for statistical analyses.

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