Mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and housed in pathogen-free facilities at Howard University. C57BL/6 (wild type; WT) male and female mice 8–12 weeks of age were used as a source of bone marrow and spleen. WT mice were additionally used for in vivo challenge experiments. All animal procedures were performed in accordance and approved by the Institutional Animal Care and Use Committee through the Office of Regulatory Research and Compliance at Howard University.

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