2.4. Preparation of amygdalar slices
This protocol is extracted from research article:
Sex differences in the delayed impact of acute stress on the amygdala
Neurobiol Stress, Jan 7, 2021; DOI: 10.1016/j.ynstr.2020.100292

Animals were sacrificed under isoflurane anesthesia. Brains were rapidly removed and placed in ice-cold cutting solution composed of (in mM): 204 sucrose, 11 glucose, 2.5 KCl, 1.25 NaH2PO4, 26 NaHCO3, 2 MgSO4, 2 CaCl2; constantly aerated with 95% O2 and 5% CO2, pH 7.3, 305–310 mOsm. 400 μm-thick coronal brain slices containing basolateral amygdala (~Bregma −1.5 mm to −3.5 mm) were obtained in the cutting solution using Leica VT1200S vibratome (Leica, Germany). Slices were immediately moved to artificial cerebrospinal fluid (aCSF) (containing (in mM): 126 NaCl, 10 glucose, 2.5 KCl, 1.25 NaH2PO4, 26 NaHCO3, 1 MgSO4, 2 CaCl2; equilibrated with 95% O2 and 5% CO2, pH 7.3, 305–310 mOsm) to recover at 30 ± 0.5 °C for 1 h followed by incubation at room temperature (22 ± 0.5 °C) until recording.

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