All NMR spectra were recorded with a Varian Mercury Plus 300 (300 MHz for 1H) spectrometer equipped with ATB broadband probe at 27 °C using as internal standard tetramethylsilane (TMS, 0.00 ppm) for NP samples and 3-(trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TMSP, 0.00 ppm) for liposome samples. In the last case, NMR was used to quantify the phospholipid concentration after the minicolumn filtration (see the “Liposome sample preparation” section)68. Results and information on sample preparations are reported in the “Supplementary Information” (Supplementary Table S1 and Supplementary Figure S2).

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