GO analysis for multiple datasets comparison was performed using ClusterProfiler package from Bioconductor76. List of genes for multiple comparisons were provided as input and enriched Biological Processes terms (FDR <0.05) were identified. For graphical simplication, a semantic similarity simplification was applied using GOSemSim function implemented in clusterProfiler, and top 10 more significant enriched terms were plot for each set of genes in the same plot. Significant biological processes were plotted with a dot size proportional to the gene ratio identified for each term and colour scale according to its adj. P value. Additionally, GO analysis of seqMiner generated clusters was performed using Panther applying filters previously used77 to reduce redundancy and generalization of selected terms. Independent GO analysis of male and female samples was performed using GREAT78, selecting top ten terms significantly enriched (FDR <0.05).

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