Fresh blood samples from BABL/c mice were anticoagulated with 3.2% sodium citrate and immediately centrifuged (3000 × g, 10 min, and 4 °C) to remove serum. The red blood cells (RBCs) collection procedures were performed in compliance with the relevant laws and institutional guidelines. The RBCs were diluted with sterile isotonic physiological buffer to obtain an RBC stock suspension. Aptamers, the mixture of DNA origami and aptamers, Aptarray, PEG 8000 (negative control) or triton X100 (positive control) was added to the RBC suspension in 1.5 ml tubes, respectively. After 3 h of incubation at 37 °C, each of the mixtures was centrifuged (1000 × g for 10 min). Hemolysis activity was determined by measuring hemoglobin absorption at 560 nm (A560) in the supernatant.

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