IL-1β expression levels in cell culture were determined using an ELISA kit (Enter life, UK). Cells were seeded in six-well plates at a density of 5 × 105 cells/well. Cell stimulation (normal, hyperosmolarity, CMC, α-MSH, CMC + α-MSH) was performed as above. After 24 h, cell supernatants were collected for ELISA analysis. ELISA procedures were performed in accordance with the manufacturer’s instructions.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.