The cytotoxicity of the origami-aptamer assemblies was assessed with a cell-counting kit (cck-8, Dojindo, Japan) containing a highly water-soluble tetrazolium salt (WST-8) [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt]. After seeding 96-well plates and culturing overnight, the HEK293T and bEnd.3 cells were incubated with Aptarray (final concentrations ranging from 0 to 56 nM and diluted with culture media) for 24 h before being washed with PBS. Next, the cells were incubated with fresh serum-free medium containing 0.5 mg/ml WST-1 for 1 h at 37 °C for the cytotoxicity assay. Absorbance values at 450 nm were measured using a microplate reader (TECAN, infinite M200, Switzerland).

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