HCECs were seeded in 12-well plates at a density of 2.5 × 105 cells/well (five groups, six wells per group). After hyperosmolarity treatment, cells were washed twice and 100 μL of lysis buffer were added to each well; cells were detached from the plate surface with cell scraper and incubated on ice for 10 min; all remaining procedures were performed in accordance with the instructions of the Caspase-1 Detection Kit (Abcam). Data were processed by subtracting the background value of each well and ratios of protein concentrations were calculated.

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