To detect total histone protein levels by western blot, the cell pellet was directly lysed in 4x SDS loading dye (400 mM Tris-HCl pH 6.8, 10% SDS w/v, 40% Glycerol, 4 mM EDTA, 400 mM DTT and 0.1% bromophenol blue) and incubated at 95°C for 15 min. The samples were diluted to 1x SDS loading dye and separated on 12% Bis-Tris Protein Gels (Life Technologies, #NP0342BOX) with NuPAGE MES SDS Running Buffer (Life Technologies, #NP0002). Antibodies are listed in Supplementary Table 3. All western blots shown in figures are representative of at least 3 independent experiments. Quantification by densitometry was performed using Image Studio Lite software.

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