All cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA) unless stated otherwise. Human embryonic kidney 293T cells (HEK293T), mouse brain endothelial cells (bEnd.3) were maintained in high-glucose DMEM supplemented with 10% FBS, 100 U/ml penicillin and 100 U/ml streptomycin. Cell line authentication was performed by short tandem repeat DNA profiling and comparison with a reference database. The cells were cultured at 37 °C, 5% CO2 and were routinely tested for mycoplasma contamination.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.