HCECs were seeded in a transwell dish (Corning, USA) at a density of 3.3 × 104 cells/well; each transwell was 6.5 mm in diameter, with a 0.4-μm filter diameter. Resistance was detected by a resistance tester at 2-day intervals; the medium in the upper and lower chambers was replaced during each measurement. Before resistance measurement, electrode calibration was performed. The long end of the electrode was passed through the space beside the upper chamber and pressed against the lower chamber vertically; the short end of the electrode was then inserted into the medium of the upper chamber without touching the bottom of the chamber. Stable readings were recorded and each measurement was repeated three times. Resistance values were recorded as the reading minus the background value.

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