Firstly, citrate anticoagulated whole blood (320 μl) from healthy donors was mixed with 35 μl samples and kaolin (10 μl) to initiate the clotting process. The four different treatments were TAE-Mg2+ buffer, the mixture of aptamers and origami (Ori + H + T, aptamers 20 µM, origami ~560 nM), Aptarray (~560 nM) and antidote-neutralized Aptarrays (fivefold antidotes pre-incubated Aptarray for 5 min). After the addition of CaCl2 (20 μl, 0.2 M), the mixture was immediately transferred to a TEG cup, and the assay was run at 37 °C according to the manufacturer’s instructions. Clot formation was measured with a Thromboelastograph Analyzer (Haemonetics) until a stable clot was formed (~60 min). The lag time, α angle and the maximum amplitude were automatically calculated by TEG analytical software 4.2.3 (Haemonetics).

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