Fresh blood from healthy volunteers was collected and plasma was prepared by centrifuging citrated human blood at 2000 × g for 15 min at 4 °C to remove blood cells. The clotting reaction of human plasma was monitored by an automatic coagulation analyzer. Thirty microliters Aptarray (with or without antidote treatment) were mixed with 270 µl plasma at 25 °C for 5 min. Buffer, TBA15, HD22, Ori + T + H, heparin and LMWH solutions, were also incubated with plasma as control groups. Activated partial thromboplastin time (APTT) assays and prothrombin time (PT) were performed using a Sysmex CS-5100 System analyzer (Siemens, Germany) according to the manufacturer’s instructions.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.