Fresh blood from healthy volunteers was collected and plasma was prepared by centrifuging citrated human blood at 2000 × g for 15 min at 4 °C to remove blood cells. The clotting reaction of human plasma was monitored by an automatic coagulation analyzer. Thirty microliters Aptarray (with or without antidote treatment) were mixed with 270 µl plasma at 25 °C for 5 min. Buffer, TBA15, HD22, Ori + T + H, heparin and LMWH solutions, were also incubated with plasma as control groups. Activated partial thromboplastin time (APTT) assays and prothrombin time (PT) were performed using a Sysmex CS-5100 System analyzer (Siemens, Germany) according to the manufacturer’s instructions.

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