For DNA origami structures, 10 µl of sample was deposited onto a freshly cleaved mica and left to absorb to the surface for 10 min. The sample was subsequently added with 40 µl TAE-Mg2+ buffer and then covered by the liquid cell for imaging. AFM imaging of DNA nanostructures before and after aptamer loading was performed in ScanAsyst mode (Mutimode-8, Bruker). The images were collected and processed using Bruker NanoScope Analysis 1.9 software.

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