Mice were released in the corner of a 45 × 45 cm open field arena (Fig. 3a). Their activity was recorded with a camera mounted on the ceiling above the center of the open field arena for 10 min. At the end of testing, mice were returned to their home cage. The arena was partitioned into outer periphery and center region, and the amount of time spent and the distance travelled were extracted using a previously validated open-source toolbox for automated phenotyping of mouse behaviour44.

Infusion of β-adrenergic receptor agonist at—0 h do not modulate innate anxiety levels in 2-month-old APP/PS1 mice: (a) Open-field test apparatus. WT and APP/PS1 mice were tested for distance travelled (b), time spent in the center (c), and time spent in the outer periphery (d). n = 5; two-way ANOVA followed by Sidak’s post hoc test. Data are presented as mean ± S.E.M.

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