Ten microliters of 0.1% sodium fluorescein were dripped into each rat’s inferior conjunctival sac. Three artificial blinks were performed and fluorescein staining of the corneal surface was observed under a slit-lamp microscope with cobalt blue light. The cornea was divided into four quadrants and each quadrant was scored as follows, according to the staining degree and area of fluorescein: 0, no staining; 1, scattered punctate staining (fewer than 30 punctae); 2, punctate staining (more than 30 punctae), but not dispersive; 3, serious dispersive staining, but no plaque formation; 4: plaque staining. The sum of the four quadrants was recorded as the corneal fluorescein staining score.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.