Ten microliters of 0.1% sodium fluorescein were dripped into each rat’s inferior conjunctival sac. Three artificial blinks were performed and fluorescein staining of the corneal surface was observed under a slit-lamp microscope with cobalt blue light. The cornea was divided into four quadrants and each quadrant was scored as follows, according to the staining degree and area of fluorescein: 0, no staining; 1, scattered punctate staining (fewer than 30 punctae); 2, punctate staining (more than 30 punctae), but not dispersive; 3, serious dispersive staining, but no plaque formation; 4: plaque staining. The sum of the four quadrants was recorded as the corneal fluorescein staining score.

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