Calu-3 and HBEC6-KT cells were cultured as described previously41,42,71,72. Cells were pretreated with IBMX (20 μM) for 2 h prior to experimental conditions. After the pre-treatment of IBMX, cells were treated with ABCC4 inhibitors (0.01–100 μM) or DMSO for 30 min. Following these exposures, the cells were treated with forskolin (10 μM) for 6 h and cell-culture supernatants were collected for analysis of extracellular cAMP. The negative control was IBMX alone and the positive control was IBMX and forskolin.

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