Forty female Wistar rats (6–8 weeks of age, 160–180 g) were purchased from the Chinese Academy of Military Medical Sciences (Beijing, China). The animals were housed at 25 °C ± 1 °C with 40% ± 5% humidity under 12-h light–dark illumination cycles. The animals were provided food and water ad libitum. The rats were randomly divided into normal, DE, CMC, and CMC + α-MSH groups. The DE, CMC, and CMC + α-MSH groups were injected subcutaneously with 6 mg/mL scopolamine hydrobromide, four times per day for 28 days. This approach has been demonstrated to successfully induce DE in rats11,26. Rats in the CMC group received topical treatment with 0.5% CMC (Allergan, Dublin, Ireland) eye drops, twice per day; rats in the CMC + α-MSH group received 1 × 10−3 mg/mL α-MSH (Millipore US) dissolved in 0.5% CMC eye drops, twice per day. The treatment regimen was initiated on the first day of the modelling protocol. The SIt and corneal fluorescence staining were performed at 7-day intervals. On the 28th day, eyeballs were collected from all rats. These experimental procedures were approved by the Experimental Animal Ethic Committee of Tianjin Medical University (SYXK 2009-0001) and adhered to the tenets of the Association for Research in Vision and Ophthalmology and the Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.