The trial was conducted using two replications arranged as a randomized complete block design (RCBD). On each replication, six plants of each of the 20 F3 families were grown in 15-cm pots. In addition, six plants per replication for each parent ‘Okeechobee’ and ‘Valmaine’ were planted as susceptible and resistant controls. Due to highly tedious work involved in L. trifolii colony maintenance, mating pair collection, and stippling damage evaluation, the experiments were conducted using two replications.

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