Lipids accumulation in the cells was monitored by staining intracellular lipid granules with oil red O (ORO) stain. Briefly, the cells were fixed in 4% PFA for 45 min, washed with PBS and stained with ORO stain (0.5%) for 30 min at RT. After washing with distilled water, cells were observed under Leica DM750 microscope (Leica Microsystems, Germany). For quantitative analysis, the accumulated ORO stain was extracted in 50% isopropanol and absorbance was recorded at 495 nm using Synergy HTX Multimode Microplate Reader (BioTek Instruments Inc., USA).

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