The nucleotide and amino acid sequences of SaAACT and SaHMGS were analyzed by bioinformatics methods, and their physical and chemical characteristics, transmembrane domain, signal peptide, and subcellular localization were predicted by corresponding bioinformatics software. Sequence assembly was performed with DNAstar ( Nucleotide sequences, deduced amino acid sequences and ORFs were analyzed, and sequences were compared through a BLAST database search ( Protein molecular weight and theoretical isoelectric point, instability index, aliphatic index and grand average of hydropathicity were calculated by ExPASy ( Protein conserved domains and active sites were predicted by the Conserved Domains database in NCBI ( and by Prosit ( in ExPASy. Transmembrane domains were analyzed on the TMHMM Server ( Signal peptides were analyzed by SignalP ( Protein subcellular localization was predicted by PSORT II (

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