To examine the effect of IMD 0354 on the growth of HaCat (HPV−ve), HeLa (HPV 18 +ve), CaSki and SiHa (both HPV 16 +ve), the cells were plated out and allowed to adhere overnight. Next day their culture medium was replaced and different concentrations of IMD 0354 (Santa Cruz Biotechnologies, Santa Cruz, CA) (final concentrations 250, 500, 750, 1000, 1250 nM, using DMSO to equalize input volumes and as negative control 0 nM) were added to the plates. Cells were counted 48 h later using a haemocytometer. The assay was repeated 3 times. One-way ANOVA test using the Prism program was used for statistical analysis.

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