The experimental procedures were approved by the institutional review board of Kitasato University (approved number: B18-265), and University of Toyama (Approved Number: 25–44) and signed informed consent for the use of samples was obtained from each patient. PFMΦs were purified as previously described35. Culture of primary ESCs was described elsewhere7. Human samples were taken and used under each patient’s consent. All experimental methods were carried out in accordance with relevant guidelines and regulations of Declaration of Helsinki.

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