Descriptive statistics included mean and standard deviation (SD) for normally distributed variables, with normality tested by inspection of histograms and using Shapiro–Wilk test. Student t-test was conducted to examine differences in ocular and OCT parameters between better eyes and worse eyes, with better eye defined as the eye with better SAP MD. The relationship between OCT and OCTA parameters and potential confounders including age and OCT scan quality score was also explored using univariate regression analyses. Parameters that are found to be significant on univariate regression analyses were further investigated using multivariable regression analyses to determine whether relationships remained after accounting for the effect of age and scan quality.

Regression analyses assume residuals are independent, however, there is likely to be correlation between eyes of an individual. To avoid loss of data from excluding eyes, analyses were conducted comparing worse and better eyes and regression analyses was performed with standard errors adjusted to take into account observations within individuals being non-independent. Standard errors were calculated based on aggregate scores from individual patients using the STATA ‘cluster’ option31. All statistical analyses were performed in STATA (version 15.1; StataCorp LP, College Station, TX). The alpha level (type I error) was set at 0.05.

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