Total RNA and cellular protein were isolated using a miRvana PARIS kit (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions. Protein expression was examined via western blotting as described previously52. cDNA was prepared using PrimeScript 2 first-strand cDNA synthesis kit (Takara Bio, Otsu, Japan). RT-qPCR was preformed using SYBR Green PCR master mix (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer's instructions. All quantitative data were normalised against expression levels of β-actin. miR-494-3p expression was analysed by RT-qPCR using a TaqMan miRNA assay kit (002365; Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer's instructions. U6 small RNA was used as the internal control for miR quantification (001973; Thermo Fisher Scientific, Waltham, MA, USA). Primer sets used in this study are listed in Supplementary Table S1.

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