Confluent cultures of A10 cells and HAoSMCs in non-coated 96 well black, clear bottomed plates were deprived of serum for 16 h and then incubated in Hank’s Balanced Salt Solution (HBSS) with Fluo-4 acetoxymethyl ester (Fluo-4 AM) (Dojindo Molecular Technologies, Kumamoto, Japan) at 37 ℃ for 30 min. Fluo-4 AM-loaded cells were washed three times with HBSS, then suprabasin-derived peptides were applied. Cell fluorescence was read at the indicated times with an excitation wavelength of 485 nm and an emission wavelength of 535 nm using a POWERSCAN HT microplate reader (BioTek Instruments, Winooski, VT, USA)8,50.

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