Confluent cultures of A10 cells and HAoSMCs in non-coated 96 well black, clear bottomed plates were deprived of serum for 16 h and then incubated in Hank’s Balanced Salt Solution (HBSS) with Fluo-4 acetoxymethyl ester (Fluo-4 AM) (Dojindo Molecular Technologies, Kumamoto, Japan) at 37 ℃ for 30 min. Fluo-4 AM-loaded cells were washed three times with HBSS, then suprabasin-derived peptides were applied. Cell fluorescence was read at the indicated times with an excitation wavelength of 485 nm and an emission wavelength of 535 nm using a POWERSCAN HT microplate reader (BioTek Instruments, Winooski, VT, USA)8,50.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.