This study was approved by the Animal Care and Use Committee of the Shiga University of Medical Science (Otsu, Japan). All animal experiments were carried out in accordance with the Guidelines for the Husbandry and Management of Laboratory Animals of the Research Center for Animal Life Science at Shiga University of Medical Science. Male C57BL/6J mice (8-week-old) were obtained from Charles River Japan (Yokohama, Japan) and maintained on a chow diet with ad libitum access to water. Mice were maintained in environmentally controlled rooms.

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