Values are expressed as means ± SEM. The un-epithelialized area and blood flow were modeled with relation to the treatment group and time by mixed effects ANOVA. Differences between groups at each time point were estimated by Hommel-adjusted contrasts using R statistical software55. Other analyses were performed by paired t-test with GraphPad Prism version 7.0c for Mac OS X (GraphPad Software, CA). The significance level was set at P < 0.05.

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