Macrotermes barneyi colony harboring all castes and fungus combs was collected from Zhongshan, Guangdong Province in southern area of China (113° 27″ E, 22° 48″ N). The colony was carefully wrapped and brought to the laboratory. After arriving the lab, the dead termite and broken combs were removed, and the complete fungus combs with healthy termites were put into a customized plastic container, which was kept in incubator at 27°and 85% humidity . The termite species was identified by the morphology of the soldier and mitochondrial COII gene sequencing30.

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