The following two methods were used to estimate kg. The slope method—the following Eq. (6) derived from Eq. (2) shows that the slope of the line connecting a point and the origin is kgAG.

Estimating the slope of the regression line through the origin by the least square model:

where xi, yi are MRBC and iA1c1000-23iA1c of each participant, respectively.

The direct method—the kg of each participant was calculated by the following equation:

Then, average and standard deviation of each kg was calculated.

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