Green and red cultivars of roselle were grown at the Medicinal Plant and Economic Development Research Center (MPED) experimental farm, Crop production farm, the University of Fort Hare, located at 32∘46′47′′ S and 26∘50′5′′ E and altitude of 524 m at East Alice in the Eastern Cape South Africa. Seeds of roselle were obtained from the Institute of Agricultural Research and Training (IAR&T) Ibadan, Nigeria. The identity of the seeds was confirmed in the herbarium of the institute.

Roselle leaves were harvested at pre-flowering, flowering and post-flowering stages of growth while calyces and seeds were harvested when mature. These were washed with water, chopped into smaller pieces, oven-dried at 40 °C until constant weights were obtained. The dried samples were then blended into a powdery form. The samples and the following analyses were managed and carried out under the same conditions for homogeneity. All the samples tested were in triplicates.

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