In a typical mix of reactions, 5 mL of C. bulbosa tuber extract is diluted with 45 mL of double distilled water (DDW), followed with the addition of 20 mL of 40 mM Selenous acid solution. The mixture solution was stirred for 24 h at 37 °C (room temperature) until the color changes from yellow to ruby red. In the end the resultant product is washed with DDW by centrifugation at 10,000 rpm for 10 min. The washing step was repeated several times until the impurities were removed. Finally, the red pellet was dried in freeze drier for two days and used for further study.

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