MEK-inhibited tail fragments were treated with light therapy in order to investigate the possibility of rescuing regeneration at the original applied R-wound. After initial trail experiments using different wavelengths, the full spectrum of visible light (VIS) was used for the therapy. SCHOTT KL 1500 lcd with a halogen lamp, color temperature of 2950 K and light intensity of 6.500 lx was used to apply light therapy to the MEK-inhibited fragments. Samples were treated with VIS light for two times 1 h each separated by 1 h of recovery in full darkness. Control animals were kept in the dark. Light intensity was measured using the LI-COR quantum/radiometer/photometer (model LI-189), in exactly the same experimental setup as used for the light therapy. All treated samples were handled very carefully in order to prevent wounding.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.