MEK-inhibited tail fragments were treated with light therapy in order to investigate the possibility of rescuing regeneration at the original applied R-wound. After initial trail experiments using different wavelengths, the full spectrum of visible light (VIS) was used for the therapy. SCHOTT KL 1500 lcd with a halogen lamp, color temperature of 2950 K and light intensity of 6.500 lx was used to apply light therapy to the MEK-inhibited fragments. Samples were treated with VIS light for two times 1 h each separated by 1 h of recovery in full darkness. Control animals were kept in the dark. Light intensity was measured using the LI-COR quantum/radiometer/photometer (model LI-189), in exactly the same experimental setup as used for the light therapy. All treated samples were handled very carefully in order to prevent wounding.

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