Dormant, MEK-inhibited fragments were exposed to H2O2 (Sigma Aldrich, 30% (v/v) in water) with the intention to rescue regeneration. After initial range finding experiments, fragments were exposed to either 1.5 mM (0.005%) or 2.25 mM (0.0075%) H2O2 (in cultivation medium) for 6 h. Afterwards they were washed for 3 times and kept in fresh medium. All treated samples were handled very carefully in order to prevent wounding.

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